secondary antiserum (Vector Laboratories)

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Vector Laboratories secondary antiserum
Secondary Antiserum, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 6670 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/secondary+antiserum/Biotinylated+Rabbit+Anti-Rat+IgG+Antibody/pm40696219-109-6-36
Average 96 stars, based on 6670 article reviews

secondary antiserum - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Reproductive experience influences the effects of Lactocaseibacillus rhamnosus HN001 on gut microbiota and hippocampal plasticity in female rats
Article Snippet: Tissue was incubated overnight at 4°C in rabbit anti‐DCX 1:5000 (Abcam, ab18723), mouse anti‐synaptophysin 1:500 (Sigma‐Aldrich, 5768), rabbit anti‐Iba1 1:5000 (Wako #19‐19741), or mouse anti‐Fos 1:500 (Abcam, ab208942). .. Sections were incubated in an appropriate secondary antiserum (1:500 biotinylated donkey anti‐rabbit or 1:500 biotinylated goat‐anti‐mouse) for 2 h at room temperature followed by processing using the avidin–biotin–peroxidase complex (Vectastain® Elite® ABC HRP Kit #PK‐6100; 1:1000; Vector Laboratories) and DAB (DAB kit; Vector Laboratories). .. Sections were mounted on Superfrost Plus slides (Fischer Scientific, Pittsburgh, PA), dried, dehydrated, and coverslipped with Permount (Fischer Scientific, Pittsburgh, PA).

Article Title: Reproductive experience influences the effects of Lactocaseibacillus rhamnosus HN001 on gut microbiota and hippocampal plasticity in female rats.
Article Snippet: Tissue was incubated overnight at 4 C in rabbit anti-DCX 1:5000 (Abcam, ab18723), mouse anti-synaptophysin 1:500 (Sigma-Aldrich, 5768), rabbit anti-Iba1 1:5000 (Wako #19-19741), or mouse anti-Fos 1:500 (Abcam, ab208942). .. Sections were incubated in an appropriate secondary antiserum (1:500 biotinylated donkey anti-rabbit or 1:500 biotinylated goat-anti-mouse) for 2 h at room temperature followed by processing using the avidin–biotin–peroxidase complex (Vectastain® Elite® ABC HRP Kit #PK-6100; 1:1000; Vector Laboratories) and DAB (DAB kit; Vector Laboratories). .. Sections were mounted on Superfrost Plus slides (Fischer Scientific, Pittsburgh, PA), dried, dehydrated, and coverslipped with Permount (Fischer Scientific, Pittsburgh, PA).

Avidin-Biotin Assay:

Immunohistochemistry:

Article Title: Temporal Patterns of Fos Expression in the Dentate Gyrus after Spontaneous Seizures in a Mouse Model of Temporal Lobe Epilepsy
Article Snippet: .. The protocol was similar to that for Fos immunohistochemistry, with replacements of appropriate normal serum (horse), primary antiserum (1:1000; mouse anti-NeuN; catalog #MAB377; Chemicon International, Temecula, CA), and secondary antiserum (1:200; biotinylated horse anti-mouse IgG from Vector Laboratories). ..

other:

Article Title: Effects of the group I metabotropic glutamate receptor agonist, DHPG, and injection stress on striatal cell signaling in food-restricted and ad libitum fed rats
Article Snippet: Following several PBS washes, sections were incubated with a secondary antiserum (Vector, Burlingame, CA) for 60 min and subsequently reacted with avidin-biotin complex (ABC) (Vector).

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